Other

Part:BBa_K1624008:Design

Designed by: Andrew Ellington   Group: iGEM15_LASATX   (2015-09-16)


pCoof->RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR'd out T7 and lacO from pCDF plasmid, replacing with pCoof promoter, in front of RBS.


Source

BBa_K352002 (pCoof), BBa_K1624003 (RBS). From lab member, pCDF plasmid.

References